The presentation of PCS-500-010

PCS-500-010 is activated to ensure the beef can be passaged at atomic three times (i.e., about 9 to 10 citizenry doublings) afterwards thaw in complete advance
media (Mesenchymal Axis Corpuscle Basal Medium additional one
Mesenchymal Axis Corpuscle Advance Kit–Low serum) and abutment
differentiation.Each lot of PCS-500-010 is activated to ensure in
vitro adverse to adipocytes, chondrocytes and osteocytes.
**A Certificate of Analysis (COA) is accessible aloft appeal for anniversary lot
of cells. Greater than 95% of the corpuscle citizenry expresses these markers by
flow cytometry. Less than 2% of corpuscle citizenry expresses these markers by breeze
cytometry. While the ability flasks equilibrate, abolish one canteen of
PCS-500-010 from accumulator and thaw the beef by affable agitation in a 37°C baptize bath. To abate the achievability of contamination, accumulate the O-ring and cap out of the water. Thawing should be accelerated (approximately 1 to 2 minutes)One canteen of Umbilical Cord-Derived Mesenchymal Axis Cells; Normal, Human (ATCCTrypsin-EDTA for Primary Beef PCS-999-003) absolute 0.05% Trypsin and 0.02% EDTA.
Note: Do not use added trypsin-EDTA concentrations PCS-500-010) absolute a minimum of 5 x 105viable beef (provided).The beef are cryopreserved in the additional access to ensure the accomplished activity and plating efficiency.
Mesenchymal Axis Corpuscle Basal Medium, if supplemented with Mesenchymal Axis Corpuscle Advance Kit - Low serum components, provides an ideal corpuscle arrangement to bear mesenchymal axis beef in low serum (2% FBS) conditions. If maintained beneath optimal advance conditions, ATCC Normal Human Umbicial Cord-Derived Mesenchymal Axis Beef accept been apparent to be multipotent, able of appropriate down the adipogenic, osteogenic and chondrogenic lineages.
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